Chromatographic Methods for the Determination of Rifampicin, Isoniazid, Pyrazinamide, Ethambutol, and Main Metabolites in Biological Samples: A Review

Abstract

Bioanalytical methods are used to quantify drugs and their metabolites in biological samples in order to determine bioequivalence, perform pharmacokinetic and bioavailability studies, and complete therapeutic drug monitoring. The objective of this review paper is to describe bioanalytical methods based on Liquid Chromatography that are used to quantify antitubercular drugs and their metabolites in different biological samples, utilizing scientific literature from 1992 to 2021.

Keywords:

• Bioanalytical
• chromatographic method
• liquid chromatography
• mass spectrometry
• tuberculosis drug

Acknowledgments

The authors thank the National University of Trujillo, which through the CANON MINERO 2022 fund, subsidized the publication of this study. Thanks to Dr. Charles Peloquin and Dr. Nicole F. Maranchick of the University of Florida for reviewing the English grammar of this article.

Declaration of competing interest

The authors declare no conflict of interest related to the topic described in this review.