A Review on Spike and Recovery Method in Analytical Method Development and Validation

Abstract

In multidisciplinary science, Analytical approaches based on spike and recovery (SAR) play a substantial role in analytical testing. The spike and recovery method is an important technique for analyzing and accessing the accuracy of analytical methods. The goal of this review seeks to provide clarity on the role of SAR methods in the forensic science discipline. Recent literature has been searched from numerous databases like Google, Web of Sciences, Scopus, PubMed, Google Scholar, and SciFinder. Websites like Science Direct are critically explored to gather scientific reports related to SAR utility. This review discusses the applications and current role of the SAR methods in Forensic Toxicology. It is suggested as one of the major parameters in the validation of the analytical method. SAR methodology is extremely important for the identification and quantitation of analytes in the sample matrix. Moreover, the extension of SAR methods to any scientific discipline is equally important for quality assurance. All relevant processes like method development and its optimization, quality control, and assurance rely on SAR-based studies. However, the method requires better apprehension and needs to be utilized using standard guidelines.

Keywords:

• Spike
• Recovery
• Method Validation
• Quality Assurance
• Forensic Toxicology

Acknowledgment

The first author acknowledge the award of Senior Research Fellowship (SRF) granted by the University Grant Commission (UGC), Ministry of Education, Government of India.

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

No funding was received to assist with the preparation of this manuscript. The authors have no relevant financial or non-financial interests to disclose.