Abstract
Cell culture-based vaccine technology is a flexible and convenient approach for vaccine production that requires adaptation of the vaccine strains to the new cells. Driven by the motivation to develop a broadly permissive cell line for infection with a wide range of viruses, we identified a set of the most relevant host receptors involved in viral attachment and entry. This identification was done through a review of different viral entry pathways and host cell lines, and in the context of the Baltimore classification of viruses. In addition, we indicated the potential technical problems and proposed some solutions regarding how to modify the host cell genome in order to meet industrial requirements for mass production of antiviral vaccines. Our work contributes to a finer understanding of the importance of breaking the host–virus recognition specificities for the possibility of creating a cell line feasible for the production of vaccines against a broad spectrum of viruses.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Author contributions
XFD selected the topic, initiated the project, conceptualized the ideas, and drafted the manuscript. XFD and XZ prepared the figures and tables. LA introduced critical references and revised the manuscript. All authors contributed to literature search, analysis, interpretation and manuscript preparation. KO coordinated the collaboration among authors. All authors have critically reviewed, discussed, and approved all the content of the manuscript for publication. The authors would like to thank Mackenzie Waltke for proofreading this manuscript.