Abstract
Intraoral fixed appliances remain in the potentially corrosive environment of the mouth for an average of two years. Over time, corrosion causes the release of metal ions, such as nickel and chromium. These metals can become allergenic and cytotoxic, causing different conditions in the human body. The aim of this study therefore is to carry out a systematic review of the available scientific evidence on the accumulation of metal ions, and the genotoxic and cytotoxic effects in oral mucosa cells deriving from short- and long-term exposure to them. The systematic review is reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The primary outcome (quantification of metal ion deposits and assessment of their genotoxic and/or cytotoxic effects) and secondary outcome (complementary analysis of cytotoxic and genotoxic effects) were examined. The Cochrane Collaboration tool and Toxicological data Reliability Assessment Tool (ToxRTool) were used for quality assessment. Once the search was performed, a total of seven articles met the inclusion criteria and were included in this study. Two main techniques were used to assess genotoxic effects: alkaline comet assay (6/7) and micronucleus method (1/7). Cytotoxicity was evaluated (4/7) using the trypan blue dye test. Accumulations of nickel (7/7), chromium (5/7), and other metals (zinc, cobalt, iron, manganese, molybdenum, titanium) were also quantified. The results allowed us to conclude that release of metal ions and acute cell and DNA damage in oral mucosa cells takes place in the early stages of treatment. However, more long-term studies are needed to evaluate chronic exposure to metals and DNA damage, as well as cellular capacity to recover DNA integrity.
Acknowledgments
The authors would like to gratefully acknowledge the Department of Clinical Specialties (DECO) of the Complutense University of Madrid for the support for the English editing of the final version of this manuscript. The authors would like to acknowledge and thank the anonymous reviewers selected by the Editor for their useful and constructive comments that have improved the final version of the paper.
Declaration of interest
No potential competing interest is reported by the authors. The authors assume all responsibility for the content reflected in this work. The authors' work affiliations are listed on the title page. All authors have contributed to the preparation of this review. None of the authors of this work has appeared in any legal proceedings related to the content of this article. This article has not received external funding support.
Supplemental material
Supplemental material for this article is available online https://doi.org/10.1080/10408444.2021.1960271.