An updated perspective on the polymerase division of labor during eukaryotic DNA replication

Abstract

In eukaryotes three DNA polymerases (Pols), α, δ, and ε, are tasked with bulk DNA synthesis of nascent strands during genome duplication. Most evidence supports a model where Pol α initiates DNA synthesis before Pol ε and Pol δ replicate the leading and lagging strands, respectively. However, a number of recent reports, enabled by advances in biochemical and genetic techniques, have highlighted emerging roles for Pol δ in all stages of leading-strand synthesis; initiation, elongation, and termination, as well as fork restart. By focusing on these studies, this review provides an updated perspective on the division of labor between the replicative polymerases during DNA replication.

Keywords:

• DNA replication
• replisome
• DNA polymerase
• polymerase delta
• replication fork

Disclosure statement

No potential conflict of interest was reported by the author(s).

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by the Medical Research Council as part of United Kingdom Research and Innovation [MRC grant No. MC_UP_1201/12 to J.T.P.Y]. T.A.G. is supported by a Sir Henry Wellcome Postdoctoral Fellowship from the Wellcome Trust [213596/Z/18/Z].