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ABSTRACT
A combination of GC and MS was used to identify 40 different compounds in the essential oil of Thuja occidentalis L. foliage samples collected from mid-Michigan (USA). The essential oils were obtained by three different methods: pilot scale steam distillation, cool hexane solvent extraction, and laboratory steam microdistillation. By all three methods the major compounds identified were α-thujone (39–56%), fenchone (6.0–15.0%), β-thujone (7.2–11.0%), sabinene (2.1–8.9%), beyerene (1.30–5.95%), bornyl acetate (1.20–4.38%) and camphor (1.5–3.3%). Fifteen diterpenes were found, eight of which were identified for the first time in the oil of T. occidentalis foliage. Variations in the oil composition as a function of isolation technique are also reported.