Volatile and non-volatile metabolite study of endemic ecuadorian specie Piper lanceifolium Kunth

ABSTRACT

Phytochemical study of P. lanceifoluim, used in the traditional medicine in the South of Ecuador, resulted in the isolation of one flavone called Sakuranetin (1), two benzoic derivative acid knows called: lanceaefolic acid methyl ester (2), and cyclolanceaefolic acid methyl ester (3). The structures of compounds were established by means of ¹H- and ¹³C-NMR and MS data, and compared with literature data. The essential oils were obtained by hydrodistillation and then analysed by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS), a total of 35 constituents were identified, representing the 97.3% of the chemical composition. Safrole was the most abundant compound in the plant with 48.3%. Monoterpene hydrocarbons and oxygenated monoterpenes are the most important groups of compounds in the essential oil of P. lanceifolium, this oil exhibited a moderate antibacterial effect against Klebsiella pneumoniae (ATCC 9997).

KEYWORDS:

• Piper lanceifolium
• lanceaefolic acid methyl ester
• cyclolanceaefolic acid methyl ester
• Sakuranetin
• essential oil
• ecuador

Acknowledgments

Financial support for this study was granted by the “Universidad Técnica Particular de Loja” (PROY_QUI_1237). We thank the Ministerio del Ambiente Ecuador for providing the “Contrato Marco de acceso a Recursos Genéticos MAE-DBN-CM-2016-0048”.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.