Physical study of proton therapy at CANAM laboratory on medulloblastoma cell lines DAOY

Abstract

2.0 MeV proton beam accelerated at Tandetron is extracted in air through a thin film and allowed to scatter to irradiate the cell culture attached to the polymeric base of a biological flask. The irradiated cells were human medulloblastoma cell line Daoy treated with and without 5 nm sized spherical gold nanoparticles. Proton doses from 0.5 to 1.5 Gy have been employed to irradiate the cultures and to investigate the role of the radiotherapy performed with and without the use of the gold nanoparticles. Results indicated that cell survival is significantly reduced to about 50% when the nanoparticles at a concentration of about 6 × 10¹³ particles/ml are employed.

KEYWORDS:

• Protontherapy
• Bragg peak
• medulloblastoma cell line DAOY
• proton dose

Acknowledgements

The research has been realized at the CANAM (Center of Accelerators and Nuclear Analytical Methods) infrastructure LM 2011019 and has been supported by project GACR 19-02482S. This publication was supported by OP RDE, MEYS, Czech Republic under the project CANAM OP, CZ.02.1.01/0.0//0.0/16_013/00018.12.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The research has been realized at the CANAM (Center of Accelerators and Nuclear Analytical Methods) infrastructure LM 2011019 and has been supported by project No. P108/12/G108.