Influence of radiation dose from repeated chest X-ray on cell morphology and proliferation in peripheral blood mononuclear cells an in vitro study

Abstract

The chest X-ray is a simple technique for routine medical examinations. Patients under investigation (PUI) might be exposed to radiation over the recommended dose due to the necessity of repeated X-rays. The aim of this research was to study the effects of repeated chest X-rays on peripheral blood mononuclear cells (PBMCs). The study determined characteristics of PBMCs by inverted microscope and flow cytometry after single and repeated X-ray exposure doses. The morphology of PBMCs showed cell differentiation to mesenchymal stromal cells throughout the plate and the trend of cells to differentiate in control groups more than single exposure and repeated irradiation. The cell growth of PBMCs was decreased after 10 days following repeated X-ray exposure as compared to controls. The overall results indicated that low-dose radiation from diagnostic X-rays affected PBMCs possibly indicating harmful radiation exposure. Future clinical studies will necessitate a larger sample size to more clearly assess cellular changes.

KEYWORDS:

• Repeated X-ray
• peripheral blood mononuclear cells
• chest X-ray
• biomarkers
• low-dose radiation

Acknowledgements

We thank Associate Professor Manus Mongkolsuk, Dean of the Faculty of Radiological Technology, Rangsit University for their assistance.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This project was financially supported by scholarships supported from Research Institute of Rangsit University.

Notes on contributors

Gunjanaporn Tochaikul

Gunjanaporn Tochaikul is a Lecturer in Faculty of Radiological Technology, Rangsit University, Patumthani, Thailand.

Chalermchai Pilapong

Chalermchai Pilapong is a Lecturer in Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.

Krai Daowtak

Krai Daowtak is a Lecturer in Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, Thailand.

Nutthapong Moonkum

Nutthapong Moonkum is a Lecturer in Faculty of Radiological Technology, Rangsit University, Patumthani, Thailand.