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DNA Sequence Volume 17, 2006 - Issue 1
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Cloning, Characterization And Tissue-specific Expression Of A Cdna Encoding A Novel Embryonic Flower 2 Gene (Osemf2) In Oryza Sativa

Kegui Li School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
,
Jinshui Yang School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
,
Jun Liu School of Life Sciences, Shanghai University, Shanghai, P.R. China
,
Xiling Du School of Life Sciences, Shanghai University, Shanghai, P.R. China
,
Chun Wei School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
,
Wei Su School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
,
Guangming He School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
,
Qinghua Zhang School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
,
Fang Hong School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
&
Xiaoyin Qian School of Life Sciences, Fudan University, Institute of Genetics, State Key Laboratory of Genetic Engineering, Shanghai, P.R. China
 show all
Pages 74-78 | Received 21 Jan 2005, Published online: 11 Jul 2009
 
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Abstract

EMBRYONGIC FLOWER 2 (EMF2) gene plays a major role in maintain vegetative development and repress flower development. Here, we present the cloning, characterization and tissue-specific expression of a putative EMF2 (OsEMF2) gene in Oryza sativa. The full-length cDNA of OsEMF2 was 1899 bp and contained an 1872 bp open reading frame (ORF) encoding a 624 amino acid protein. Homologous analysis showed that OsEMF2 contain a single conserved C2H2-type zinc finger motif. Sequence alignment shows that there is a homology between the deduced amino acid sequence of OsEMF2 and EMF2 in Zea mays (55%). Moreover, pI of OsEMF2 are predicted. The tissue-specific expression pattern of OsEMF2 reveals that it is abundant in shoot apical meristem and inflorescence meristem, while its expression level is much lower in leaf, root, immature seed and callus.

Keywords

Acknowledgements

This work was supported by the Chinese 973 Foundation (Grant No.2001CB108805).

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