Isolation and characterization of a new Na⁺/H⁺ antiporter gene OsNHA1 from rice (Oryza sativa L.)

Abstract

The full-length cDNA (3612 bp) of OsNHA1 was cloned by RT-PCR approach from rice (Oryza sativa L.), which encodes a putative plasma membrane Na⁺/H⁺ antiporter. Its deduced protein, OsNHA1, has 11 transmembrane domains and a significant similarity to a plasma membrane Na⁺/H⁺ antiporter AtNHA1 from Arabidopsis thaliana. Phylogenetic analysis showed that the OsNHA1 clusters with the plasma membrane Na⁺/H⁺ antiporters from various organisms. The semi-quantitative RT-PCR assay revealed that the expression of OsNHA1 was up-regulated in both shoots and roots of rice seedlings under salt stress, whereas it was not induced in the rice seedlings treated by drought stress.

• Rice
• Na⁺/H⁺ antiporter
• gene cloning and expression
• salt stress

Acknowledgements

This work was supported by grants from Ph. D program foundation from Ministry of Education (20050307013) and the National Transgenic Plant Research and Commercialization Special Project of China (JY03A1101)

Notes

^*Sequence data from this article have been deposited in GenBank/EMBL/DDBJ under Accession Number: AY328087