Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus

Abstract

In study of gene expression profile in cloned embryos which derived from D. rerio embryonic nuclei and G. rarus enucleated eggs, cytochrome c oxidase subunit I (COXI) of G. rarus, exhibiting difference at expression level between cloned embryos and zebrafish embryo, was cloned. Its full cDNA length is 1654 bp and contains a 1551 bp open reading frame, encoding a 5.64 kDa protein of 516 amino acids. The alignment result shows that mitochondrion tRNA^ser is co-transcripted with COXI, which just was the 3′-UTR of COXI. Molecular phylogenic analysis based on COXI indicates G. rarus should belong to Gobioninae, which was not in agreement with previous study according to morphological taxonomy. Comparison of DNA with cDNA shows that RNA editing phenomenon does not occur in the COXI of G. rarus.

• Cytochrome c Oxidase Subunit I
• RNA editing
• Gobiocypris rarus
• Danio rerio

Acknowledgements

The authors are grateful to Ming Li for supplying experimental materials. This work was supported by the State Key Fundamental Research of China (Grant Nos. G2000016109 & 2004CB117406), the National Natural Science Foundation of China (Grant No. 90208024) and the Chinese Academy of Sciences (Grant No KSCX2-SW-303).