Identification of the genes GPD1 and GPD2 of Pichia jadinii

Abstract

Two genes coding for proteins with a high degree of sequence similarity to glycerol-3-phosphate dehydrogenases have been isolated from the yeast Pichia jadinii. Fragments of the genes were PCR-amplified with degenerated primers from genomic DNA of P. jadinii. Clones containing the full-length genes PjGPD1 and PjGPD2 were isolated by screening genomic libraries. DNA sequencing revealed open reading frames (ORFs) of 1182 bp and 1185 bp for PjGpd1p and PjGpd2p, respectively. In a complementation study PjGPD1 rescued the growth defect of a Saccharomyces cerevisiae Δgpd1 mutant strain under osmotic stress, while complementation by PjGPD2 is temperature sensitive.

The sequences of the PjGPD1 and PjGPD2 ORFs have been submitted to the EMBL Nucleotide Sequence Database under Accession No. AJ632339 and AJ632340, the sequences of the corresponding genomic DNA fragments under Accession No. AJ632341 and AJ635370, respectively.

• Pichia jadinii
• Saccharomyces cerevisiae
• glycerol-3-phosphate dehydrogenase

• AJ632339
• AJ632340
• AJ632341
• AJ635370