Investigation on Interaction of L-Methionine Dipeptide with ct-DNA by Ultraviolet Spectroscopy

Abstract

In this article, L-methionine peptides were synthesized with the assistance of phosphorus oxychloride. L-methionine dipeptide was purified by HPLC and characterized by the means of ¹ H-NMR, ¹³ C-NMR and ESI-MS. The interaction of L-Methionine dipeptide with ct-DNA was studied by ultraviolet spectra. The results showed that L-Methionine dipeptide could interact with phosphorous groups of ct-DNA. The influences of interaction time, ionic strength, and phosphate anion on the interaction between L-methionine dipeptide and ct-DNA were also investigated.

Keywords:

• ct-DNA
• interaction
• L-methionine dipeptide
• Phosphorus oxychloride
• ultraviolet spectra

Acknowledgments

The authors would like to thank the financial supports from the Chinese National Science Foundation (No. 20272055, 20572016), the Henan Province Science Foundation for Prominent Youth (No. 0312000900), and the Office of Education of Henan Province (No. 2006KYCX017).