Preparation of Adrenochrome Hydrogel Patch, Gel, Ointment, and the Comparison of Their Blood Coagulating and Wound Healing Capability

ABSTRACT

The aim of this study is to make an effective blood coagulant and wound healing agent, which on its topical application on ruptured skin would help in instant coagulation of blood and ongoing healing of wound. The hydrogel has been prepared by mixing 28% w/v gelatin and 21% w/v PVA in distilled water, and heated to 40°C followed by addition of a blood coagulant at a lower temperature. Beeswax, alcohol, liquid paraffin, and adrenochrome were mixed, triturated, and heated accordingly to prepare adrenochrome ointment. Polyvinyl alcohol and glycerin were mixed and heated and the drug was added at a lower temperature, and stored at 4–5°C to form adrenochrome gel. Gelatin alone has cell adhesion property. Adrenochrome is a blood coagulant. Therefore, gelatin with adrenochrome in hydrogel has a synergistic effect in wound healing. To evaluate the efficacy of these three different formulations, incisions were made on the backs of three mice and simultaneously adrenochrome containing hydrogel patch, gel, and ointment were applied on the wound and observed at regular intervals for half an hour to examine the rate of blood coagulation and kept under observation for 2 days to study the rate of wound healing. The efficacy of all these three formulations was compared to appraise the most effective blood coagulating and wound healing agent.

Keywords:

• Adrenochrome monosemicarbazone
• Blood coagulation
• Capillary fragility
• Controlled release
• Cytotoxicity
• Drug delivery
• Gel
• Hemostatic effect
• Hydrogel patch
• Hydrophilic polymers
• Ointment
• Synergistic effect
• Thromboplastin
• Transdermal
• Wound healing

Notes

Hydrogel is observed to release drug for 12 hours at nearly zero-order rate.