ABSTRACT
The objective of this work was to evaluate the use of Myrcia sylvatica Hydrolate (MSH) as a sedative under conditions of simulated transport of Colossoma macropomum juveniles. Simulated a 17 h transport of the load densities 30, 60, and 90 fish/L, with 2.5%, 5%, and 7.5% MSH and water-only control (W.C.). There was no significant difference in survival, temperature, hardness, and total and non-ionized ammonia between treatments and densities. The pH decreased as MSH concentration increased. Dissolved oxygen was lower and CO2 was higher in the end transport in relation initial. Alkalinity and total dissolved solids increased significantly as a function of density. We found lower levels of conductivity and ion flux (sodium, potassium, and Chloride) and a lower number of neutral and acidic gill mucous cells for 5% MSH at the highest bulk density. Therefore, suggest that 5% MSH may be an alternative sedative for the transport of tambaqui juveniles.
Acknowledgments
The Amazonia Paraense Foundation for Research Support (FAPESPA) for funding and granting a scholarship; the Secretariat of Agricultural Development of Fisheries of the State of Pará-SEDAP for the logistical support for the experiments, together with its technical staff at the pisciculture station of Santa Rosa. To LABBEX and Laboratory of Multiuser Chemistry (UFOPA) for support in the analyses.
Disclosure statement
No potential conflict of interest was reported by the authors.
Ethics committee
This project was approved by the Research Ethics Committee of the State University of Pará (Campus XII, Santarém) Registration no. 42/2012. All animal manipulations were performed in accordance with the guidelines of the ethics committee mentioned above.