Antioxidant and antibacterial efficacy of Chaetomorpha linum and its toxicological evaluation for the prophylactic treatment against Pseudomonas aeruginosa infection in Labeo rohita

ABSTRACT

The antioxidant activity and antibacterial efficacy of aqueous and ethanolic extracts of Chaetomorpha linum were tested against bacterial infection caused by Pseudomonas aeruginosa. In vitro and in vivo studies revealed the efficacy of ethanolic extract of C. linum in controlling the P. aeruginosa infection. Gas Chromatography Mass Spectroscopy analysis of purified fractions of ethanolic extract was also performed. Ichthyotoxic bioassay of purified seaweed extracts was performed to assess its toxic effect in rohu fish and found to be nontoxic. Experimental pathogenicity of P. aeruginosa showed exophthalmia, reduced intake of feed, and lesions on the body. LD 50 was achieved at the 3.96 × 102 at 48 h and 4.82 × 103 at 72 h in the experimental pathogenicity. The results revealed that the ethanolic extract of C. linum can be used as an alternate source for controlling P. aeruginosa infection in L. rohita.

KEYWORDS:

• Aquaculture disease management
• bacterial infection
• active compounds
• fish toxicity
• in vivo treatment

Acknowledgments

The authors thank the Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (formerly known as Saveetha University) for providing the facilities to carry out this research work.

Disclosure statement

We do not have any known conflicts of interest in this work.

Ethical approval

The use of fishes in the study does not require ethical approval.