Different dietary protein levels for Podocnemis unifilis subadult farming: hematological and biochemical assessment

ABSTRACT

This study evaluated the physiological responses of subadult yellow-spotted Amazon river turtle, Podocnemis unifilis, to incremental crude protein levels (29%, 32%, 35%, and 39% CP) as extruded commercial feeds. The hematological and plasma biochemical parameters of P. unifilis were analyzed at 60, 120, and 180 days of the feeding trial. The increase in total thrombocyte, leukocyte, lymphocyte, heterophil, and eosinophil numbers, besides high respiratory burst activity showed an improvement in animal immune defense response to incremental protein in diets, acting together to maintain the integrity of the tissues against antigens and infectious agents. Elevated subadult P. unifilis plasma total cholesterol and triglyceride levels observed after increasing the feeding time (until 180 days) with incremented protein levels in artificial diets during captivity are a nutritional warning, related to the condition of being less physically active. Based on these results, periodic physiological evaluations are particularly important to ensure the healthy and adequate nutritional conditions of captive-bred animals, such as maintaining lower stocking densities of animals to avoid stress and, if possible, providing them with fresh food in addition to extruded artificial feed.

KEYWORDS:

• Leukocytes
• glucose
• triglyceride
• cholesterol
• health status

Acknowledgments

The authors thank the Graduate Program in Tropical Biodiversity (PPGBIO) at Federal University of Amapá (UNIFAP); CAPES for providing the Master’s scholarship to A. R. P. Brasiliense; and Brazilian Agricultural Research Corporation in Amapá (Embrapa Amapá), for infrastructure and logistical support. This work was supported by Banco da Amazônia (Process number 03.16.00.058.00.00).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors reported there is no funding associated with the work featured in this article.