Expression Profiles of Different Cytokine Genes in Peripheral Blood Mononuclear Cells of Goats Infected Experimentally with Native Strain of Mycobacterium Avium Subsp. Paratuberculosis

Abstract

Paratuberculosis (ParaTB), caused by Mycobacterium avium subspecies paratuberculosis (MAP) is a chronic enteritis of ruminants and may contribute to Crohn's disease in humans. Key features of host immunity to MAP infection include an early pro-inflammatory (Th1-like) response that eventually gives way to a predominant anti-inflammatory (Th2-like) response. Many studies have been conducted to understand the underlying mechanism of misdirected host immune response, however, these studies mainly focused on cattle. The present study is the first attempt to test the hypothesis of shift in Th1 to Th2 like responses during the progression of ParaTB in caprine species (small ruminant). Ten healthy male kids (<6 months old) of the same breed were selected for this study. Of the 10 kids, 6 were experimentally infected with native strain (S5) of MAP (“Indian Bison Type”) and the remaining 4 kids were control. Kids were monitored for a period of 12 months post infection (MPI) and were tested for establishment of infection. Expression levels of IFNG, IL2, IL12, IL4, and IL10 genes were estimated before infection and at 4, 8, and 12 MPI in stimulated peripheral blood mononuclear cells (PBMCs) of infected and control kids. The study demonstrated the expression of IFNG and IL2 as classic Th1-like pro-inflammatory signatures; whereas, IL10 exhibited itself as classical Th2-like signature. The study also reports unexpected lowered expression of the IL12 gene simultaneously with increased expression of IFNG, lowered expression of the IL2 gene (compared to IFNG), and suppressed expression of the IL4.

Keywords:

• Cytokine
• Goat
• Mycobacterium avium subspecies paratuberculosis
• paratuberculosis

Acknowledgments

P. K. Singh and A. V. Singh are currently affiliated with the National JALMA Institute for Leprosy & Other Mycobacterial Diseases, Tajganj, Agra (UP), India. J. S. Sohal is currently affiliated with the Canadian Food Inspection Agency, 3400 W Casavant, St-Hyacinthe, Canada J2S 8E3.

Sincere thanks to Council of Scientific and Industrial Research (CSIR), New Delhi for awarding the “Senior Research fellowship” to P. K. Singh.

Notes

*Kids having “Strong positive” status in S/P ratio were considered as positive for MAP infection; ^#Number of kids in experimental infection group was 6.