Analysis on Differential Expressed Genes of Ovarian Tissue Between High- and Low-Yield Laying Hen

Abstract

In order to elucidate molecular genetic mechanism of laying hen reproduction at the transcriptional level and the structure of significantly differential genes, the mRNA differential display and reverse northern dot-blot were used to detect the differential expression of genes in the ovary tissue of low-yield laying hens and high-yield laying hens in the present study. Sixteen 32-week-old CAU-pink laying hens divided into two groups were used and the laying performance was measured. The results showed that only the egg numbers were significantly different between the two groups; and from 15 primer pairs, a total of 336 bands were displayed of which 59 cDNA bands were found to be differentially expressed in both high-yield and low-yield laying hen. The sequence analysis indicated that the expression of such bands as H-AP5, H-P5, and H-P4 was significantly potentiated in high-yield laying hen using primer pairs AP5/HT11G, P5/HT11G and P4/HT11G and these transcripts had high homology (98%) to HoxDb, HoxCa, and HoxBa, respectively. The differentially expressed gene fragments may be relevant to the progression of the high-yield hens to the egg-laying stage. And further study is required to elucidate the molecular function to improve the productivity of laying hens.

Keywords:

• CAU-pink laying hen
• Gene expression
• mRNA differential display
• Ovary tissue

Acknowledgments

W. Chen and L.-J. Song contributed equally to this work.

The authors appreciate the financial support provided by Agricultural Elite Breeds Project of Shandong Province.

Notes

The data shown in table are arithmetic mean ± standard deviation (n = 8).

^{a , b} Means within the same row with different superscript indicate significant difference (P < 0.05).

Abbreviations: Ne: normal-expressed bands; Oe: over-expressed bands; Ae: absence-expressed bands; Se: specific-expressed bands; Ce: co-expressed bands.