LncRNA-000133 from secondary hair follicle of Cashmere goat: identification, regulatory network and its effects on inductive property of dermal papilla cells

Abstract

Long noncoding RNAs (lncRNAs), a class of non-protein conding RNAs > 200 nt in length, were thought to play critical roles in regulating the expression of protein-coding genes. Here, we identified and characterized a novel lncRNA-000133 from the secondary hair follicle (SHF) of cashmere goat with its ceRNA network analysis, as well as, its potential effects on inductive property of dermal papilla cells were evaluated through overexpression analysis. Expression analysis indicated that lncRNA-000133 had a significantly higher expression at anagen than that at telogen in SHF of Cashmere goat, suggesting that lncRNA-000133 might be involved in the reconstruction of SHF with the formation and growth of cashmere fiber. Taken together with methylation analysis, we showed that 5’ regulatory region methylation of the lncRNA-000133 gene might be involved in its expression suppression in SHF of Cashmere goat. The ceRNA regulatory network showed that a rich and complex regulatory relationship between lncRNA-000133 and related miRNAs with their target genes. The overexpression of lncRNA-000133 led to a significant increasing in the relative expression of ET-1, SCF, ALP and LEF1 in dermal papilla cells suggesting that lncRNA-000133 appears to contribute the inductive property of dermal papilla cells.

Keywords:

• LncRNA-000133
• SHF
• regulatory network
• inductive property
• dermal papilla cells
• Cashmere goat

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Additional information

Funding

The work was supported financially by grants from National Natural Science Foundation of China (No.31672388, No.31872325, and No.31802038), Natural Science Foundation of Liaoning Province, China (No. 2015020758), Key Project Foundation of Education Department of Liaoning Province, China (No. LSNZD201606), Innovative Talent Support Program Foundation of Universities and Colleges in Liaoning Province, China (LR2016035), Science and Technology Innovation Talent Support Foundation for Young and Middle-aged People of Shenyang City, China (RC170447), Tianzhushan Yingcai foundation of Shenyang Agricultural University (2015, Shenyang, Liaoning, China).