ALDH1A1 effect on Yan Yellow Cattle preadipocyte differentiation

Abstract

Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cytosolic enzyme that mainly catalyzes the oxidation of acetaldehyde into acetic acid and participates in the regulation of differentiation and gene expression in fat cell growth and development. However, the physiological role of ALDH1A1 in the formation of fat cell precursors in the Yan Yellow Cattle is still not clear. Herein, we investigated the specific regulation of the gene encoding for ALDH1A1 during the differentiation process of the adipocyte cells of the Yan Yellow Cattle by interfering or overexpressing the ALDH1A1 gene. As a result, we found that the mRNA expression levels of ALDH1A1 were significantly increased during the formation of progenitor cells. In addition, the expression levels of the Lipoprotein lipase (LPL) and transcription factors (PPARγ, C/EBPα) were also significantly increased. ALDH1A1 gene overexpression and RNA interfering promoted and inhibited respectively the lipid accumulation and triglyceride production in mature adipocytes, and the expression of the LPL and transcription factors (PPARγ, C/EBPα). The changes in the protein expression levels of ALDH1A1 and adipogenic factors were in accord with the changes observed in the mRNA levels. In conclusion, our results indicate that ALDH1A1 plays an important regulatory role in the differentiation of preadipocyte cells.

Keywords:

• Aldehyde dehydrogenase1A1
• adipogenesis
• lipid accumulation
• triglyceride

Author contributions

Z. H. and Y. C. designed the experiments; Y. C. and Y. Z. supported the experimental materials; Z. H. and Y. C. analyzed the data; Z. H. wrote the manuscript and the manuscript was modified by Y. C. and Z. H., Y. Z., H. J., J. W., L. Q., and C. X. supervised the project.

Disclosure statement

The authors have declared that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Additional information

Funding

This work was supported by Jilin Provincial Academy of Agricultural Sciences Innovation Project [CXGC2017JQ001], National Beef Cattle Industry Technology System [CARS-37], Key Science and Technology R&D Project of Jilin Province Science and Technology Department: Key Technology Development and Demonstration to Improve Beef Production and Quality in High-end Parts [20180201039NY], Domestic animal germplasm resources platform, and by Jilin Province Science and Technology Development Plan Project [20160204006NY].