Novel polymorphism of HSP70 gene affected caprine physiological vital parameters

Abstract

The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar) which differ in body size, coat color and production performance were assessed for effect of thermal stress at different temperature humidity index (THI). The physiological vital parameters like rectal temperature (RT), skin temperature (ST), heart rate (HR) and respiration rate (RR) which are related to thermal stress susceptibility were recorded. The genetic variation of HSP70 gene in the three goat populations and its effect on physiological vital parameters related to thermal stress was assessed. A novel polymorphism (C241T) in the exonic region of HSP70 gene with significant (p < .05) statistical association with the physiological vital parameters like RT, ST and RR was identified indicating its role in thermo-tolerance.

Highlights

• The HSP70 gene was found to be polymorphic in Indian goats.

• The non-synonymous nucleotide change (C241T) resulted change in amino acid Arginine 241 Cysteine in mature polypeptide which were not reported earlier.

• The constructed phylogenetic tree showed that Ganjam and Raighar goats are more close to each other.

Keywords:

• Gene
• goat
• stress
• temperature
• thermo-tolerance

Acknowledgments

The authors are thankful to the authority of Odisha University of Agriculture and Technology for providing necessary facilities to carry out the research work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The necessary funding for the research work was provided by the Indian Council of Agricultural Research (ICAR) Extramural Project, New Delhi.