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Animal Biotechnology Volume 32, 2021 - Issue 5
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Articles

The expression of miR-129-5p and its target genes in the skin of goats

Jialu Lia College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China;b Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China;c Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Beibei, Chongqing, P. R. ChinaView further author information
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Lingbin Liua College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China;b Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China;c Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Beibei, Chongqing, P. R. ChinaView further author information
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Jipan Zhanga College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China;b Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China;c Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Beibei, Chongqing, P. R. ChinaView further author information
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Lei Chenga College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China;b Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China;c Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Beibei, Chongqing, P. R. ChinaView further author information
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Lingtong Rena College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China;b Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China;c Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Beibei, Chongqing, P. R. ChinaView further author information
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Yongju Zhaoa College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China;b Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China;c Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Beibei, Chongqing, P. R. ChinaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0001-9256-8856View further author information
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Pages 573-579 | Published online: 20 Feb 2020
 
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Abstract

Coat color is one of the major quality traits of animals, and miR-129-5p acts as an important regulator for melanin biosynthesis in mammals. In this study, real-time PCR and western blotting were used to examine the expression of miR-129-5p and its targets genes in the skin of different coat color goats. The results showed that the expression of miR-129-5p in the skin samples of Inner Mongolia cashmere goats (IMCG) was higher than that of Dazu black goat (DBG). Also, the target genes (tyrosinase (TYR), frizzled 6 (FZD6) and glycogen synthase kinase 3β (GSK3β)) of miR-129-5p was highly expressed in the skin samples of DBG. The expression of miR-129-5p firstly increased and then decreased with age in F1 hybrid generation of DBG and IMCG. In addition, the expression of TYR decreased with age, while the expression of MITF increased with age but then decreased. The expression of FZD6 and GSK3β in the skin samples of F1 of different ages were irregular. Our results indicated that miR-129-5p mainly affects the formation of coat color of goats by decreasing the expression of TYR. This study suggests that miR-129-5p can act as a suppressor in the formation of coat color to lay the foundation for studying the effect of miR-129-5p on melanin synthesis.

Ethical approval

This experiment was conducted following the International Council for the Coordination of Animal Welfare (ICCAW) guidelines for the care and use of laboratory animals. Norms concerning the ethical treatment of animals during the study were strictly followed.

Disclosure statement

No potential conflict of interest was reported by the author(s).

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Additional information

Funding

This work was supported by National Key Research and Development Plan of China [2018YFD0502003], the National Natural Science Foundation of China [No. 31772564], Chongqing Technology Innovation and Application Development Special Project [No. cstc2019jscx-lyjsAX0018, CY190209].

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