Fermented sugarcane bagasse with Lactobacillus combined with cellulase and molasses promotes in vitro gas kinetics, degradability, and ruminal fermentation patterns compared to rice straw

Abstract

This experiment was aimed to study the effect of sugarcane bagasse (SB) fermented with Lactobacillus casei TH14, cellulase, and molasses on in vitro gas kinetics, nutrient digestibility, and ruminal fermentation patterns compared to rice straw (RS). A 2 × 2 × 2 (+1) factorial arrangement in a completely randomized design was used. Factor A was levels of L. casei TH14 at 0 and 0.05 g/kg fresh matter, factor B was levels of cellulase at 0 and 10⁴ U/kg fresh matter, and factor C was levels of molasses at 0 and 5 g of substrate DM. The (+1) treatment referred to RS fermentation without additives. The results showed that kinetics of gas, gas production, and total volatile fatty acid were not different between RS and SB treatment. RS had significantly (p < 0.05) greater nutrient digestibility, ruminal pH, ammonia-nitrogen, and protozoa compared the control SB. Compared with control RS and SB, SB fermented with additives had greater (p < 0.05) gas from soluble fraction and rate constant of gas, in vitro dry matter and neutral detergent fiber digestibility, and ruminal propionate. In conclusion, SB fermented with L. casei TH14, cellulase, and molasses in combination promoted ruminal gas production, in vitro digestibility, and ruminal fermentation patterns.

Keywords:

• Lactobacillus
• gas production
• ammonia-nitrogen
• methane production
• volatile fatty acids

Acknowledgements

The authors would like to thanks to Tropical Feed Resources Research and Development Center (TROFREC) and Department of Animal Science, Faculty of Agriculture, Khon Kaen University for research facilities; Khon Kaen Sugar Industry Public Company Limited (KSL) for kind support of SB.

Disclosure statement

No conflict of interest was reported by the author(s).

Additional information

Funding

Acknowledge to the Increase Production Efficiency and Meat Quality of Native Beef and Buffalo Research Group, KKU, and the Royal Golden Jubilee PhD Program for Asian (Contract No. PHD/0159/2561) and Office of Coordination for Sugarcane and Sugar Research Project (Contract No. RDG62T0081) under Thailand Science Research and Innovation (TSRI), Khon Kaen University (KKU) for providing financial support.