Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case–control study in the Indian cattle population

Abstract

This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical body condition score, which was then screened by a panel of diagnostic tests viz. Johnin, ELISA, fecal microscopy, and fecal culture, for the establishment of a case–control resource population. A total of four SNPs viz. rs208222804, rs211654540, rs208814257, and rs210748127 in CD209 gene were genotyped by PCR-RFLP. All SNPs, except rs210748127, were polymorphic in our population. Genotypic–phenotypic associations were assessed by the PROCLOGISTIC procedure of SAS 9.3. The SNP rs208814257 yielded three genotypes viz. CC, CG, and GG, which were significantly (p < 0.05) different in case as compared to the control population. The odds of CC and CG in comparison to GG genotype were 1.21 and 0.40, respectively. The CG genotype was significantly higher in control population, indicating that this genotype may provide resistance against PTB in our resource population. Upon validation in an independent, larger test population and following biological characterization, SNP rs208814257 can be incorporated in marker panel for selection of animals with greater resistance to MAP infection.

Keywords:

• Paratuberculosis
• SNPs
• CD209
• immune response
• genotype
• resistance

Acknowledgments

Authors are thankful to the Director, ICAR-Indian Veterinary Research Institute, Izatnagar, India for providing necessary facilities and funding of this work with grant no. IXX09774.

Statement of animal rights

The experiments performed on animals had prior approval of Institutional Animal Ethics Committee (IAEC) of ICAR-Indian Veterinary Research Institute, Izatnagar, U.P. India, and in accordance with the guidelines of Committee for the Purpose of Control and Supervision of Experimentation in Animals (CPCSEA), Ministry of Environment, Forest and Climate Change, Government of India.

Disclosure statement

The authors do not have any conflict of interest.