Angiopoietin-like protein 8, molecular cloning and regulating lipid accumulation in goat intramuscular preadipocytes

Abstract

This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study the effect of ANGPTL8 on the differentiation of goat intramuscular preadipocytes. The total length of the ANGPTL8 gene nucleotide sequence is 717 bp, including 597 bp of ORF, encoding 198 amino acids. Goat ANGPTL8 has the closest relationship with sheep, it was widely expressed in different tissues, and relatively enriched in liver. The silence of ANGPTL8 inhibited the accumulation of lipid droplets by 5.76% in goat intramuscular preadipocytes (p > 0.05) and significantly suppressed the expression of the genes related to preadipocytes differentiation, fatty acid synthesis and transport (p＜0.05 or p＜0.01). These data illuminate the speculation that ANGPTL8 may involve in the lipid accumulation regulation via the control of PPARγ and C/EBPβ in goat adipocytes.

Keywords:

• Jianzhou goat
• intramuscular preadipocytes
• ANGPTL8
• lipid metabolism

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was jointly supported by National Key R&D Program of China [Beijing, China; 2018YFD0502002 and 2016YFC0500709], the National Natural Science Foundation of China [Beijing, China; 31601921 and 31672395], Sichuan Science and Technology Program [Sichuan, China, 2020JDJQ0010], Science Fund for Distinguished Young and Middle-aged Scholars of National Ethnic Affairs Commission (Beijing, China), Fundamental Research Funds for the Central Universities, Southwest Minzu University [Beijing, China, 2020PTJS17].