Determination of the relationship between Anatolian black cattle growth properties and myostatin, GHR and Pit-1 gene

Abstract

The aim of this study is to determine the relationship between some growth and development characteristics in Anatolian Black cattle from birth to twelve months of age with the Pit-1, GHR and Myostatin genes. PCR-RFLP method was used to detect the polymorphism. Genotype and allele frequencies were found to be AA/AB/BB: 0.096/0.519/0.385 and A/B: 0.356/0.644; AA/AG/GG: 0.346/0.385/0.269 and A/G: 0.538/0.462 in the Pit-1 and GHR genes respectively. Myostatin gene was found to be also monomorphic in all genotypes. Although the chi-square χ² test in the Pit-1 gene showed an agreement to Hardy–Weinberg equilibrium (p > 0.05), in the GHR gene did not showed an agreement (p < 0.05). The results of the statistical analysis indicated an association between Pit-1 and GHR genes polymorphism and growth traits at different stage ages in Anatolian Black cattle. But Pit-1/HinfI gene and GHR/Alul polymorphisms were not found statistically significant in the specified periods, at all characters. On the other hand, since the MSTN/BstF5I gene was found to be monomorphic, no association analysis was performed between the measured values and this gene. In conclusion, mutation of these genes is difficult to suggest as a potential marker in a herd selection regarding the growth and development characteristics.

Keywords:

• Anatolian Black cattle
• GHR
• myostatin
• PCR-RFLP
• Pit-1

Acknowledgment

This study was prepared from the doctoral dissertation of Çağrı Melikşah SAKAR, who is the first name in the article. We would like to thank ‘Turkey’s Ministry of Agriculture and Foresty, General Directorate of Agricultural Research and Policies’ who have given the necessary permission to provide the animal material that used in the study.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

We would like to thank ‘Selçuk University, Scientific Research Projects Coordination Unit’ for supporting this study with the project numbered 18201039.