Analysis of m⁶A methylation in skin tissues of different sex Liaoning cashmere goats

Abstract

N⁶-methyladenosine (m⁶A) is the most frequent internal modification of mRNA and lncRNA in eukaryotes. We used two high-throughput sequencing method, m⁶A-seq and RNA-seq to identify pivotal m⁶A-modified genes in cashmere fineness and fiber growth. 8062 m⁶A peaks were detected by m⁶A-seq, including 2157 upregulated and 6445 downregulated. Furthermore, by comparing m⁶A-modified genes of the male Liaoning Cashmere Goat (M-LCG) and female Liaoning Cashmere Goat (F-LCG) skin tissues, we get 862 differentially expressed m⁶A-modified genes. To identify differently expressed m⁶A genes associated with cashmere fineness, 11 genes were selected for validation using real time fluorescent quantitative PCR in M-LCG and F-LCG. This study provides an acadamic basis on the molecular regulation mechanism of m⁶A modification in cashmere growth process.

Keywords:

• m⁶A-seq
• RNA-seq
• cashmere fineness
• differently m⁶A methylated genes

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

1. Grants from the National Natural Science Foundation of China (NO. 31802038). 2. The 69th batch of China Postdoctoral Science Foundation (Regional Special Support Program) (NO. 2021M693859). 3. Youth seedling project of Liaoning Provincial Department of Education, China (LSNQN201905). 4. Liaoning Provincial Department of science and technology, agricultural key issues and industrialization project (2020JH2/10200029).