Autochthonous buffalo-gut origin Pediococcus pentosaceus RM119 decreased diarrhea and enhanced gut health, immunity in neonatal Murrah calves

Abstract

This study assessed the effect of autochthonous probiotic Pediococcus pentosaceus RM119 on gut health, growth, and nutrient utilization in calves. Twelve buffalo calves (<15 d) were divided into two groups, control without probiotics, and probiotic group with P. pentosaceus RM119 @ 10⁸ CFU/calf/d. The probiotic group showed a reduction (p < 0.05) in fecal score, diarrhea episodes and duration of diarrhea. The fecal pH, fecal ammonia was lower, whereas lactate was higher in probiotic group than control. There was a significant increase (p < 0.01) in the concentration of fecal acetate, propionate and butyrate levels in the probiotic supplemented group. The fecal lactobacilli and bifidobacterium were higher (p < 0.01), whereas, fecal coliform and clostridial count were lower (p < 0.01) in P. pentosaceus RM119 supplemented group. There was an improvement in reduced glutathione anti oxidant. Overall, buffalo-gut origin P. pentosaceus RM119 reduced the frequency and severity of diarrhea in neonatal buffalo calves and improved the gut health.

Graphical Abstract

Keywords:

• Buffalo calves
• diarrhea
• immunity
• Pediococcus
• probiotic

Acknowledgments

The authors are thankful to Director, ICAR-IVRI for all the infrastructure and financial support.

Ethical approval

For all animal handling procedures, due ethical approval was taken from CPCSEA, DAHD, New Delhi vide letter no 25/17/2019-CPCSEA dated 25/9/2019.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data associated with the paper is available as supplementary material with this manuscript.

Data deposition

The probiotic P. pentosaceus RM119 MT181120.1 is deposited in NCBI.

Additional information

Funding

This study was supported by ICAR‐Indian Veterinary Research Institute, Izzatnagar, India.