Association between the cashmere production performance, milk production performance, and body size traits and polymorphism of COL6A5 and LOC102181374 genes in Liaoning cashmere goats

Abstract

The purpose of this study was to analyze the relationship between COL6A5 (collagen type VI alpha 5 chain) and LOC102181374 (alcohol dehydrogenase 1) genes and the production performance of Liaoning cashmere goats by single nucleotide polymorphism (SNP). We have searched for SNP loci of COL6A5 and LOC102181374 genes through sequence alignment and PCR experiments, and have used SPSS and SHEsis software to analyze production data. We obtained five SNP loci in total, including three SNP loci (G50985A, G51140T, G51175A) in COL6A5 gene and two SNP loci (A10067G, T10108C) in LOC102181374 gene. The genotypes G50985A (AG), G51140T (GT), G51175A (AA), A10067G (AA), and T10108C (CC) of these loci have certain advantages in improving the production performance of Liaoning cashmere goats. The haplotype combinations that can improve production performance in COL6A5 gene were H1H5:AGGGAG, H4H4:GGGGAA, and H4H4:GGGGAA. H3H3:GGCC and H2H4:AGTT were the dominant combinations in LOC102181374 gene. At G51175A and A10067G loci, we found that H1H2:AAAG and H1H3:AGAA have dominant effects. These results may provide some support for the molecular breeding of production traits in Liaoning cashmere goats.

Keywords:

• COL6A5
• LOC102181374
• SNP
• cashmere fineness
• milk fat

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

Our scientific research was financially aided by five projects: 1. 2022 National Natural Science Foundation of China General (Project No. 32272836). 2. Postdoctoral Science Foundation of China: Genetic trajectory and expression localization of key genes of cashmere fineness by multi-omics (Project No. 2021M693859). 3. 2021 Liaoning Province “the open competition mechanism to select the best candidates” Science and Technology Research Project: Selection and breeding of special advantageous livestock and poultry breeds in Liaoning and key technology of whole industry chain production (Project No. 2021JH1/10400033). 4. Shenyang Breeding Industry Innovation Special Project: Tackling-key Technology on Selection and Breeding of Fine Cashmere Liaoning Cashmere Goat (Project No. 22-318-2-07). 5. National modern agricultural industrial technology system (Project No. cars-39-27).