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Animal Biotechnology Volume 1, 1990 - Issue 2
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Original Articles

Successful production of transgenic rats

Shin‐ichi Hochi Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., 519 Shimoishibashi, Ishibashi‐machi, Shimotsuga‐gun, Tochigi, 329‐05, Japan
,
Takashi Ninomiya Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., 519 Shimoishibashi, Ishibashi‐machi, Shimotsuga‐gun, Tochigi, 329‐05, Japan
,
Miho Honma Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., 519 Shimoishibashi, Ishibashi‐machi, Shimotsuga‐gun, Tochigi, 329‐05, Japan
&
Atsushi Yuki Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., 519 Shimoishibashi, Ishibashi‐machi, Shimotsuga‐gun, Tochigi, 329‐05, Japan
Pages 175-184 | Published online: 23 Sep 2009
 
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Abstract

DNA of pSV2‐gpt‐gE1A or SV2‐cat microinjected into pronuclei of fertilized rat eggs was found incorporated into chromosomes of 3 out of 48 (6.3%) or 10 out of 66 (15.2%) new born rats, respectively. The transgenic rats carrying SV2‐cat DNA transmitted the transgenes to their G1 progeny.

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