Cytotoxicity, Antibacterial, Antioxidant, and Antibiofilm Properties of Tunisian Juglans regia Bark Extract

Abstract

Tunisian Juglans regia bark ethanol extract (JREE) was examined for antibacterial, antibiofilm, and antioxidant activity. Its anti-proliferative effect was evaluated on normal (MRC-5) and cancer (HT29 and HEp-2) cell lines. The data revealed that JREE inhibited the growth of pathogenic bacteria (MICs value ranged from 32 to 128 μg.mL-1) and induced a strong antibiofilm effect (BIC50 was 23, 30, and 43 μg.mL-1 for Micrococcus luteus, Staphylococcus epidermidis, and Staphylococcus aureus, respectively). The JREE showed high scavenging property against 2,2-diphenyl-l-1-picrylhydrazil (DPPH) radical (IC50 = 3.5 μg.mL-1) and exhibited anti-proliferative effects with IC50 values ranging from 6.6 to 25.5 μg.mL-1.

Keywords:

• Juglans
• bark
• antibacterial
• antibiofilm
• antioxidant
• MTT
• anti-proliferative

Notes

The authors thank Dr. Samir Abbès, associate professor in the Higher Institute of Biotechnology of Beja, and Dr. Adel Rdissi, English associate professor in the Faculty of Medicine, Monastir University, Tunisia, for improving the English of this report.