Abstract
Vegetative parts (stem, leaf, and nodal explants) of Tinospora cordifolia were excised from an in vivo grown mature plant and thereafter cultured on MS medium supplemented with 2, 4-D and bark extracts of Azadirachta indica and Acacia nilotica prepared in hexane, ethyl acetate, chloroform and methanol. The best callus growth was observed on MS medium supplemented with 2, 4-D (1 mg.L−1) and A. indica bark methanolic extract (1 mg.L−1). The methanol extracts of the dried callus showed the presence of alkaloids and sterols. HPLC, HPTLC, and GC-MS analysis of callus [MS+2, 4-D (1 mg.L−1)] and callus [MS+2, 4-D (1 mg.L−1) + A. indica methanolic extract (1 mg.L−1)] were carried out for comparative study. The new-found peaks revealed the presence of methyl hexadecanoic acid with molecular weight of 270.