Antioxidant and Antimicrobial Activities of Callus Culture and Fruits of Phyllanthus emblica L.

Abstract

The antioxidant activity of the callus of Phyllanthus emblica was tested by using DPPH− radical scavenging, reducing power, superoxide anion, and metal chelating scavenging models. Digallic acid was the major compound in quantity both in vivo (2.61 ± 0.887%) and in vitro (2.89 ± 0.997%) in 6-week-old cell cultures. Digallic acid demonstrated maximum antioxidant activity against the antioxidant systems, while other compounds exhibited mild-to-moderate antioxidant activity. Among the isolated phytochemicals, putranjivain-A showed maximum activity against Escherichia coli (85 μg.mL⁻¹) and Rhizoctonia phaseoli (70 μg.mL⁻¹).

KEYWORDS:

• HPLC
• DPPH-radical scavenging activity
• reducing power
• metal chelating
• superoxide anion scavenging activity
• tannins
• flavonoids

ACKNOWLEDGEMENTS

Authors are grateful to Professor Naveen Sharma, Superior Diagnostic Centre, Jaipur for providing the pure cultures of Candida albicans and to Professor R. S. Mishra, Department of Botany, University of Rajasthan, Jaipur, India for identification of plant materials.