Inhibitory Effects of Mitrella kentii Extracts on Inflammatory Mediators’ Biosynthesis and Binding

ABSTRACT

The inhibitory effects of Mitrella kentii leaf and stem extracts on the production prostaglandin E₂ (PGE₂) and thromboxane B₂ (TXB₂) and antagonist effect on platelet-activating factor (PAF) receptor binding were evaluated. The inhibition of PGE₂ and TXB₂ productions were determined using the radioimmunoassay technique, and the inhibitory effect of PAF receptor binding to rabbit platelet was determined using the ³H-PAF as a ligand. Among the extracts tested, ethyl acetate, methanol and hexane leaf extracts, and methanol stems extract showed inhibitory effects on PGE₂ and TXB₂ productions and antagonistic effect on PAF receptor binding. M. kentii extracts may have the potential of being developed as supplements for inflammatory conditions.

KEYWORDS:

• Prostaglandin E
• thromboxane B₂
• platelet activating factor
• radioimmunoassay
• blood platelets

Acknowledgments

The authors thank the Ministry of Science, Technology and Innovation, Malaysia, for the financial support (grant nos.: 02-01-02-SF0016 and 02-01-02-SF0510).

Conflicts of Interest

The authors declare no conflicts of interest in this study.

Additional information

Funding

This work was supported by the Ministry of Science, Technology and Innovation, Malaysia [grant nos. 02-01-02-SF0016, 02-01-02-SF0510].