ABSTRACT
Aerial parts of Polystichum squarrosum (Dryopteridaceae) collected from Kumaun Himalaya, India were assessed for antioxidant potential using the methanolic extract in vitro for DPPH free radical scavenging and ferric-reducing antioxidant assays. The DPPH assay exhibited potent free radical scavenging capacity (IC50, 6.03 ± 1.41 µg mL−1), as well as ferric-reducing antioxidant activity (886.2 mg AAE gm−1 dry extract). In the antibacterial activity tests, the methanolic extract inhibited the growth of Klebsiella pneumoniae (MIC 14 ± 0.57 mm) and Agrobacterium tumefaciens (MIC 12 ± 0.7 mm). Totally, 65 phytochemical compounds were identified using gas chromatography mass spectrometry (GC-MS). Total phenolics (913.33 ± 39.23 mg GAE g−1 dry extract) and total flavonoid contents (30.0 ± 2.64 mg QE g−1 dry extract) were estimated qualitatively. These results demonstrated antioxidant and antimicrobial activities due to the presence of bioactive phytochemicals.
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Disclosure Statement
No potential conflict of interest was reported by the author(s).