Optimization of Common Cuttlefish (Sepia officinalis) Protein Hydrolysate Using Pepsin by Response Surface Methodology

Abstract

Protein hydrolysate was prepared from common cuttlefish (Sepia officinalis) by pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of the prediction model. An enzyme activity to substrate ratio (X₁), 0.04 AU/g protein; time (X₂), 85 min; and temperature (X₃), 45°C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order. All other amino acids are present in sufficient quantity as required.

Keywords:

• cuttlefish protein hydrolysate (CPH)
• Sepia officinalis
• enzyme
• amino acid