Functional Properties of Ethyl Acetate-methanol Extract of Commonly Edible Molluscs

ABSTRACT

Marine molluscs are consumed as culinary delicacies and represent a relatively untapped source of natural functional food ingredients. The bioactive potential of ethyl acetate-methanol (C₂H₅OAc/CH₃OH) extract of molluscs Uroteuthis duvaucelii, Amphioctopus marginatus, Crassostrea madrasensis, and Sepiella inermis were demonstrated using different in vitro systems. The C₂H₅OAc/CH₃OH extract of A. marginatus displayed greater 2, 2-diphenyl-1-picryl-hydrazil (IC₉₀ 1.76 mg mL⁻¹) radical quenching capacity along with potential hydroxymethylglutaryl coenzyme-A reductase (HMGCR)-inhibiting activity (IC₉₀ 1.21 mg mL⁻¹) and angiotensin converting enzyme-I inhibitory property (ACE-I) (IC₉₀ 0.87 mg mL⁻¹) compared to those displayed by other molluscs. The organic extract of S. inermis set forth significantly higher pro-inflammatory 5-lipoxygenase inhibitory property (IC₉₀ 1.96 mg mL⁻¹) along with potential antidiabetic activity as determined by dipeptidyl peptidase-4 (DPP-4) antagonistic activities (IC₉₀ 2.62 mg mL⁻¹, P < .05). Significant correlations were observed between bioactive properties and electronegative groups of organic extracts of a mollusc. The free radical quenching property of the extracts exhibited a positive correlation towards other bioactivities, implicating the role of antioxidant property of the compounds in the extract to inhibit lifestyle ailments. The present study indicated that S. inermis and A. marginatus could be utilized as functional food ingredients to combat hypertension, hyperlipidemia, and inflammation-related risks.

KEYWORDS:

• Marine molluscs
• anti-inflammatory
• anti-diabetic
• anti-hypertensive
• anti-hypercholesterolemic
• functional food ingredients

Acknowledgments

The authors are thankful to the Ministry of Science and Technology of Department of Biotechnology, Government of India, New Delhi [grant number BT/PR15757/AAQ/3/ 757/2015]. The authors thank the Director, Central Marine Fisheries Research Institute for his guidance and support. Thanks are due to the Head, Marine Biotechnology Division of Central Marine Fisheries Research Institute for facilitating the research activities.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Ministry of Science and Technology of Department of Biotechnology, Government of India, New Delhi [grant number BT/PR15757/AAQ/3/ 757/2015].