Improving Product Added-value from Shrimp (Litopenaeus vannamei) Waste by Using Enzymatic Hydrolysis and Response Surface Methodology

ABSTRACT

Improving product added-value from shrimp wastes and reducing environmental pollution are important to the shrimp aquaculture industry. Response surface methodology was employed to optimize the ratio of enzyme to the substrate (ES), temperature, time, pH to maximize hydrolysis and soluble protein (SP) content from shrimp liquid waste hydrolysate. Optimal hydrolysis conditions to obtain maximum DH (27.90%) and SP (59.76 mg/g) were ES (0.21%), temperature (58.87), time (67.76 min), and pH (7.87). Characteristic analysis of the product indicated that the hydrolysate had high chemical scores of essential amino acids for humans (0.98), tilapia (1.16), and rainbow trout (1.52), flavor amino acid-rich protein (51.96 g/100 g of protein), and total carotenoids (330.5 μg/g). Previous studies have not considered value and environmental benefits achieved through utilization of shrimp liquid waste. The results indicate that shrimp liquid waste hydrolysate can be used as a valuable feed supplement and feed attractant for aquaculture.

GRAPHICAL ABSTRACT

KEYWORDS:

• Degree of hydrolysis (DH)
• soluble protein (SP)
• enzymatic hydrolysis
• optimization
• response surface methodology (RMS)

Highlights

• Shrimp liquid waste contains essential nutrients and functional soluble compounds.

• It is the first time the systematic hydrolysis of shrimp liquid waste has been reported.

• Response surface methodology was applied for enzymatic hydrolysis optimization.

• Significantly improved quality of product was obtained using the optimized hydrolysis conditions.

• Shrimp liquid waste hydrolysate can be used as a valuable feed supplement and feed attractant for aquaculture.

Acknowledgments

The study was funded by the FIRST Project (Fostering Innovation through Research, Science, and Technology) – Ministry of Science and Technology (MOST), Vietnam. The authors are thankful to Vietnam Food Co. (VNF) for providing the samples and support during the research work. We are also grateful to APOTEC’s project team for substantial effort in undertaking the study.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Ministry of Science and Technology (MOST), Vietnam [FIRST Project Fostering Innovation through Research].