Study on Salicylanilide Analogue for Antioxidant Activity and Melanosis Prevention in Chinese White Shrimp (Fenneropenaeus chinensis)

ABSTRACT

This study investigated the effects of salicylanilide analogue NBHE for shrimp preservation. The antioxidant activity of 4-Nitro-benzoic acid 3-(2-hydroxy-benzoylamino)-propyl ester (NBHE) was evaluated using the superoxide anion scavenging ability assay. The results showed that NBHE possessed a higher inhibitory effect than that of salicylic acid but weaker than that of kojic acid and ascorbic acid. The IC₅₀ value for ascorbic acid, kojic acid, salicylic acid, and NBHE was calculated to be 9.88 ± 1.98 μg/mL, 63.10 ± 1.13 μg/mL, 328.94 ± 25.01 μg/mL, and 80.94 ± 4.20 μg/mL, respectively. The copper chelating activity of 80.04 ± 3.00% and 85.39 ± 2.97% was observed in the presence of 30 μg/mL and 35 μg/mL of NBHE, respectively. Fifty μM concentration of NBHE had no considerable cytotoxic effect in HaCaT cells. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of shrimp myofibrillar proteins (MP) showed that NBHE could effectively inhibit myofibrillar protein degradation and displayed obvious preservative effect.

KEYWORDS:

• Salicylanilide analogue
• antioxidant activity
• copper chelating activity
• cytotoxicity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

We appreciate the financial support from the Marine Economy Innovation Development Area Demonstration Project of Beihai (Bhsfs003, Bhsfs009), the Natural Science Foundation and the Key Technology R&D Program of Fujian Province (No. 2018J01132 and 2019N0032).