DNA Extracted from Byproducts of Common Carp Testis and Application in Removing Ethidium Bromide from Pollutants

ABSTRACT

In the present paper, an optimized method to extract common carp sperm DNA (csDNA) from common carp testis, the mechanism of ethidium bromide (EtBr) interaction with csDNA, as well as the removal of EtBr pollutant by csDNA was studied. The results showed that high salt extraction method was suitable for extracting csDNA. Under the optimum conditions, the yield of csDNA extraction was as high as 11.7%. In addition, the results of UV–vis spectra, fluorescence spectra, and observation with fluorescence microscope indicated that csDNA could be intercalated by EtBr to form a stable complex, which confirmed that csDNA could be applied in removing EtBr pollutant. Based on the csDNA intercalation, EtBr pollutant was removed by csDNA effectively, which was 94.13%. This work not only provides a smart method to extract high purity and quality csDNA from byproducts of common carp testis but also provides an easily available csDNA to remove EtBr from pollutants.

KEYWORDS:

• DNA extraction
• common carp testis
• application
• ethidium bromide

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Guangxi Natural Science Foundation of China [grant numbers 2018GXNSFDA281030, 2020GXNSFAA159021], and the National Natural Science Foundation of China [grant number 21966008].