ABSTRACT
Alcalase enzyme (1 mg) and ultrasound (400 W) at 0–20 min were applied to obtain sulfated polysaccharides from yellowfin skin. Applying 15 min ultrasound resulted in a 0.12% increase in the rate of recovery yield. The intact structure of polysaccharides was determined by Fourier transform infrared spectroscopy, X-ray diffractometry, and scanning electron microscope. Ultrasound improved the thermal behavior of sulfated polysaccharides by affecting their microstructure, as analyzed by DSC. The color characterization (L*, a*, and b*) of ultrasound-treated samples was found to be superior compared to the control. Applying 15 min led to 16% and 9% enhancing DPPH and ABTS radical scavenging activities along with improving ferric-reducing antioxidant power of the polysaccharide.
Acknowledgments
The authors express their acknowledgment to the Tarbiat Modares University, Iran, for funding support (IG-39804).
Disclosure statement
No potential conflict of interest was reported by the author(s).
Authors contribution
Alireza Sadeghi carried out the experimental tasks including the extraction process, characterization and evaluations, data analysis, and writing article. Masoud Rezaei provided all required support in whole aspects of the investigation such as samples and chemicals provided, designing the experiments, editing the article, and providing funds from the mentioned faculty. Samaneh Pezeshk cooperated in choosing the subject, designing the experiments, and representing academic advice in laboratory experiments. Mahshid Gharib Heidari also cooperated in laboratory experiments, data analysis, and editing article.
Data availability statement
Data will be made available on request.