Determination of genotoxic and antigenotoxic effects of wild-grown Reishi mushroom (Ganoderma lucidum) using the hen’s egg test for analysis of micronucleus induction

ABSTRACT

The micronucleus (MN) technique is commonly used for genotoxicity testing. The hen’s egg test (HET) for analysis of MN induction (HET-MN) is an inexpensive, rapid and simple genotoxicity assay that is compatible with animal protection and ethical considerations. Ganoderma lucidum (Curtis) P. Karst is known also as reishi mushroom and mushroom of immortality. It has long been used to treat disorders including fungal infections, influenza, common cold, hepatitis, diabetes, high cholesterol and cancer in many countries including China and Japan. G. lucidum strengthens the immune system and reduces the side effects of chemo- and radiotherapy. We investigated the possible genotoxic and antigenotoxic effects of the aqueous extract of wild-grown G. lucidum from Turkey using the HET-MN test. Three different doses of aqueous extract of G. lucidum, 50 µg/egg vitamin C as an antigenotoxic agent and 50 µg/egg cyclophosphamide as a genotoxic compound were injected separately or together into fertilized chicken eggs at incubation day 8. Embryonic peripheral blood smears were prepared and stained with a modified May-Grünwald-Giemsa method on incubation day 11. The frequencies of MN and nuclear abnormalities in erythrocytes were determined using light microscopy. Although the aqueous extract G. lucidum exhibited no genotoxic effect, it did exhibit an antigenotoxic effect. Our findings suggest that G. lucidum extract is a valuable natural antigenotoxic agent.

KEYWORDS:

• Antigenotoxicity
• cyclophosphamide
• egg
• Ganoderma lucidum
• genotoxicity
• hen
• micronucleus
• vitamin C

Acknowledgments

We thank Prof. Dr. Gıyasettin Kaşik and Dr. Sinan Alkan, Selcuk University Mushroom Application and Research Center, for authentication of microscopic and macroscopic characteristics of mushroom samples and for collection. This work is part of the MS thesis by Bülent Çelik.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was financed by Selcuk University, Scientific Research Foundation (BAP number: 15201099).