Romanowsky staining: history, recent advances and future prospects from a chemistry perspective

ABSTRACT

Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19^th and early 20^th centuries; it has facilitated for decades the work of biologists, hematologists and pathologists working with blood cells. Despite more than a century of studying Romanowsky staining, no systematic review has been published that explains the chemical processes that produce the “Romanowsky effect” or “Romanowsky-Giemsa effect” (RGE), i.e., a purple coloration arising from the interaction of an azure dye with eosin and not due merely to their simultaneous presence. Our review is an attempt to build a bridge between chemists and biomedical scientists and to summarize the available data on methylene blue (MB) demethylation as well as the related reduction and decomposition of MB to simpler compounds by both light and enzyme systems and microorganisms. To do this, we analyze modern data on the mechanisms of MB demethylation both in the presence of acids and bases and by disproportionation due to the action of light. We also offer an explanation for why the RGE occurs only when azure B, or to a lesser extent, azure A is present by applying experimental and calculated physicochemical parameters including dye-DNA binding constants and electron density distributions in the molecules of these ligands. Finally, we discuss modern techniques for obtaining new varieties of Romanowsky dyes by modifying previously known ones. We hope that our critical literature study will help scientists understand better the chemical and physicochemical processes and mechanisms of cell staining with such dyes.

KEYWORDS:

• Azure
• bacteria
• eosin Y
• Giemsa
• hematology
• Leishman
• malaria parasite
• May-Grünwald
• methylene blue
• microorganism
• Romanowsky staining
• Wright stain

Acknowledgments

We are grateful to Dr. Richard W. Horobin, an experienced professional in the field of Romanowsky dyes, for constructive criticism and valuable advice concerning this review.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities no. [FZSM-2023-0018].