Modified extraction and characterization of keratin from Indian goat hoof: A biocompatible biomaterial for tissue regenerative applications

ABSTRACT

The Goat Hoof Keratin (GHK) is a valuable biodegradable substance for making tissue regeneration. The protein substance from GHK powder is extracted in a clean form by using modified shindai solution technique. Electrophoreical analysis produces polypeptide chains of molecular weight of 40–45 kDa using SDS-PAGE method. FTIR spectra show the characteristic absorption band for α-helix and β-sheet peptide bonds. DSC analysis provides the GHK denaturation occurred at temperature ranging from 200–250°C and thermal degradation occurs at 211°C & 387°C using TGA. The biocompatibility of GHK examined using MTT (3(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of fibroblast cells shows 91% cell viability.

KEYWORDS:

• Hoof keratin
• protein
• natural polymer
• intermediate filament
• biocompatibility

Funding

Author thanks to DST-FIST, GOI for providing fund for biomedical research lab establishment Ref Number: SR/FST/College-189/2013 (Dated on 06/08/2014) and DBT, GOI for Providing Travel Grant to attend International Conference in USA, Ref Number: DBT/CTEP/02/201601450 (Dated on 25/11/2016).