Dielectric behaviour of neutrophil gelatinase associated lipocalin detected by alpha relaxation process

ABSTRACT

We have investigated alpha (α) relaxation process and its temperature evolution in electrical double layer (EDL) around neutrophil gelatinase-associated lipocalin (NGAL), anti-lipocalin2 (anti-LCN-2) and their conjugate in phosphate buffer. The dielectric permittivity (20 Hz to 1 MHz) of α-relaxation process for LCN-2 and anti-LCN-2 shows variation with temperature. This happens due to unfolding of various microstates of protein at measured temperature intervals. The DSC thermograms of LCN-2, anti-LCN-2 and their conjugate predict that the multiple unfolding domains are parallel to the shifts in real dielectric permittivity at a particular temperature.

KEYWORDS:

• Alpha relaxation process
• lipocalin-2
• acute kidney injury
• dielectric spectroscopy

Acknowledgments

The authors sincerely thank the Director, CSIR-National Physical Laboratory, New Delhi for continuous encouragement and interest in this work. Authors thank Dr. Souvik Maiti, (CSIR-IGIB, New Delhi) for providing assistance to perform DSC experiments at their lab. Mr. Mangeram has been acknowledged for technical assistance throughout the experimental procedures.

Funding

Author M.T. is grateful for Council of Scientific and Industrial Research (CSIR), New Delhi for providing financial assistance.