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Journal of Environmental Science and Health, Part C
Environmental Carcinogenesis and Ecotoxicology Reviews
Volume 35, 2017 - Issue 3
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Original Articles

Immunoassay approach for diagnosis of exposure to pyrrolizidine alkaloids

Na LiSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong KongSAR
,
Fan ZhangKey Laboratory of Pharmaceutics of Guizhou Province, Guizhou Medical University, Guiyang, Guizhou, China
,
Wei LianSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong KongSAR;Joint Research Laboratory for Promoting Globalization of Traditional Chinese Medicines between The Chinese University of Hong Kong and Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
,
Huali WangWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning, China
,
Jiang ZhengKey Laboratory of Pharmaceutics of Guizhou Province, Guizhou Medical University, Guiyang, Guizhou, China;Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning, China;Center for Developmental Therapeutics, Seattle Children's Research Institute, Division of Gastroenterology, Department of Pediatrics, University of Washington, Seattle, Washington, USA
&
Ge LinSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong KongSAR;Joint Research Laboratory for Promoting Globalization of Traditional Chinese Medicines between The Chinese University of Hong Kong and Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, ChinaCorrespondence[email protected] [email protected]
Pages 127-139 | Published online: 09 Jun 2017
 
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ABSTRACT

Numerous pyrrolizidine alkaloid (PA) poisoning cases have been documented worldwide. Protein covalent binding with reactive metabolites generated from metabolic activation of PAs to form pyrrole-protein adducts is suggested to be a primary mechanism of PA-induced toxicities. The present study aimed to develop antibodies for diagnosis of PA exposure. Polyclonal antibodies were raised in rabbits and proven to specifically recognize pyrrole-protein adducts regardless of amino acid residues modified by the reactive metabolites of PAs. The developed antibodies were successfully applied to detect pyrrole-protein adducts in blood samples obtained from PA-treated rats and exhibited a potential for the clinical diagnosis of PA exposure.

Funding

The present study was supported by Hong Kong Jockey Club Charities Trust Fund (JCICM-15-07), Research Grant Council of Hong Kong (GRF Grant no. 471310), One-off Funding for Joint Lab/Research Collaboration (Project Code: 3132968) by CUHK, CUHK School of Biomedical Sciences–Seed Fund for Joint Establishments, and the National Natural Science Foundation of China Grants 81430086 and 81373471 (in parts).

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