A comprehensive screening shows that ergothioneine is the most abundant antioxidant in the wild macrofungus Phylloporia ribis Ryvarden

ABSTRACT

The polar and non-polar extracts from the authenticated wild mushroom Phylloporia ribis were separated by hydrophilic interaction liquid chromatography (HILIC) and by reverse phase (RP)-HPLC, respectively. A split valve separated the eluents into two fractions for free-radical scavenging analysis and for structural identification. Forty-six compounds showed scavenging activity of the stable-free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The structures of 8 antioxidants (inosine, caffeic acid, ergothioneine, p-hydroxybenzoic acid, adenosine, 3,4-dihydroxybenzaldehyde, apigenin, and naringenin) are characterized by Mass Spectrometer. Among them, ergothioneine was the most abundant (>65%) and most active antioxidant in P. ribis.

KEYWORDS:

• Antioxidants
• free-radical scavenging
• herbal medicine
• mushroom
• Phylloporia ribis

Declaration of interest

The authors have declared no conflict of interest.

Additional information

Funding

Financial supports from China Agriculture Research System (CARS-21), the Natural Science Foundation of Shandong Province (ZR2017LH071, ZR2016YL006, ZR2013HL029), the Key Project of Shandong Province (2016GSF202014), the Shandong Province Taishan Scholar Program (Lanping Guo), and the Priority Research Program of the Shandong Academy of Sciences (Lanping Guo) are gratefully acknowledged.