Designing Gelatin Nanocarriers as a Swellable System for Controlled Release of Insulin: An In-Vitro Kinetic Study

Abstract

In the present investigation gelatin nanoparticles have been synthesized and characterized by various techniques like FTIR, scanning electron microscopy, particle size analysis and surface charge measurements. The particles were allowed to swell in phosphate buffer saline (PBS) and the influence of various factors like chemical composition of nanoparticles, pH and temperature of the swelling bath was investigated on the water intake capacity of the gelatin nanoparticles. The particles were loaded with insulin and the release kinetics of insulin was studied in PBS medium under different conditions. The effects of percent loading of drug, chemical composition of nanoparticles and pH, temperature of the release medium were examined on the release profiles of the drug and the possible mechanisms of drug transport were investigated. The chemical stability of the loaded drug (insulin) was also assessed especially under highly acidic conditions of artificial gastric juice.

Keywords:

• Biodegradable polymers
• controlled release
• oral drug delivery
• macromolecular drug delivery
• nanoparticles
• biopolymers
• insulin
• gelatin

Notes

*Data have been expressed as Mean ± S.D. of at least three determinations.

*EMF data have been expressed as Mean ± S.D. of at least three determinations.

*Swelling ratios have been expressed as Mean ± S.D. of at least three determinations.

*Swelling ratios have been expressed as Mean ± S.D. of at least three determinations.